Open AccessA Multispecies Cluster of VIM-1 Carbapenemase-Producing Enterobacterales Linked by a Novel, Highly Conjugative, and Broad-Host-Range IncA Plasmid Forebodes the Reemergence of VIM-1
Author(s) -
Gabriele Arcari,
Federica Maria Di Lella,
Giulia Bibbolino,
Fabio Mengoni,
Marzia Beccaccioli,
Guido Antonelli,
Luigi Faino,
Alessandra Carattoli
Publication year - 2020
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.02435-19
Subject(s) - citrobacter freundii , klebsiella oxytoca , enterobacter cloacae , klebsiella pneumoniae , microbiology and biotechnology , biology , plasmid , enterobacteriaceae , escherichia coli , citrobacter , carbapenem , antibiotics , gene , genetics
In this study, we investigated VIM-1-producing Escherichia coli , Klebsiella oxytoca , Klebsiella pneumoniae , Citrobacter freundii , and Enterobacter cloacae strains, isolated in 2019 during a period of active surveillance of carbapenem-resistant Enterobacterales in a large university hospital in Italy. VIM-1-producing strains colonized the gut of patients, with up to three different VIM-1-positive bacterial species isolated from a single rectal swab, but also caused bloodstream infection in one colonized patient. In the multispecies cluster, bla VIM-1 was identified in a 5-gene cassette class 1 integron, associated with several genetic determinants, including the bla SHV-12 , qnrS1 , and mph (A) genes, located on a highly conjugative and broad-host-range IncA plasmid. The characteristics and origin of this IncA plasmid were studied.