Open AccessProximal Binding Pocket Arg717 Substitutions in Escherichia coli AcrB Cause Clinically Relevant Divergencies in Resistance Profiles
Author(s) -
Martijn Zwama,
Kunihiko Nishino
Publication year - 2022
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.02392-21
Subject(s) - efflux , salmonella enterica , escherichia coli , azithromycin , microbiology and biotechnology , biology , multiple drug resistance , enterobacteriaceae , salmonella , antibiotic resistance , drug resistance , antibiotics , genetics , gene , bacteria
Recent mutations in RND efflux pumps in clinical strains have further increased multidrug resistance. We show that R717L and R717Q substitutions (found in azithromycin-resistantSalmonella enterica spp.) in theEscherichia coli efflux pump AcrB dramatically increase macrolide, as well as fluoroquinolone, resistance.