Open AccessComparative Genomic Analysis of Third-Generation-Cephalosporin-Resistant Escherichia coli Harboring the bla CMY-2 -Positive IncI1 Group, IncB/O/K/Z, and IncC Plasmids Isolated from Healthy Broilers in Japan
Author(s) -
Takahiro Shirakawa,
Tsuyoshi Sekizuka,
Makoto Kuroda,
Satowa Suzuki,
Manao Ozawa,
Hitoshi Abo,
Yukari Furuya,
Ryoko Akama,
Manabu Matsuda,
Yoko Shimazaki,
Mayumi Kijima,
Michihiro Kawanishi
Publication year - 2020
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.02385-19
Subject(s) - escherichia coli , plasmid , cephalosporin , biology , microbiology and biotechnology , virology , carriage , vaccination , genetics , gene , antibiotics , medicine , pathology
The off-label use of third-generation cephalosporins (3GCs) during in ovo vaccination or vaccination of newly hatched chicks has been a common practice worldwide. CMY-2-producing Escherichia coli strains have been disseminated in broiler chicken production. The objective of this study was to determine the epidemiological linkage of bla CMY-2 -positive plasmids among broilers both within and outside Japan, because the grandparent stock and parent stock were imported into Japan. We examined the whole-genome sequences of 132 3GC-resistant E. coli isolates collected from healthy broilers during 2002 to 2014. The predominant 3GC resistance gene was bla CMY-2 , which was detected in the plasmids of 87 (65.9%) isolates. The main plasmid replicon types were IncI1-Iγ ( n = 21; 24.1%), IncI ( n = 12; 13.8%), IncB/O/K/Z ( n = 28; 32.2%), and IncC ( n = 22; 25.3%). Those plasmids were subjected to gene clustering, network analyses, and plasmid multilocus sequence typing (pMLST). The chromosomal DNA of isolates was subjected to MLST and single-nucleotide variant (SNV)-based phylogenetic analysis. MLST and SNV-based phylogenetic analysis revealed high diversity of E. coli isolates. The sequence type 429 (ST429) cluster harboring bla CMY-2 -positive IncB/O/K/Z was closely related to isolates from broilers in Germany harboring bla CMY-2 -positive IncB/O/K/Z. pST55-IncI, pST12-IncI1-Iγ, and pST3-IncC were prevalent in western Japan. pST12-IncI1-Iγ and pST3-IncC were closely related to plasmids detected in E. coli isolates from chickens in North America, whereas 26 IncB/O/K/Z types were related to those in Europe. These data will be useful to reveal the whole picture of transmission of CMY-2-producing bacteria inside and outside Japan.