Open AccessDissemination of Extended-Spectrum-β-Lactamase-Producing Enterobacter cloacae Complex from a Hospital to the Nearby Environment in Guadeloupe (French West Indies): ST114 Lineage Coding for a Successful IncHI2/ST1 Plasmid
Author(s) -
Matthieu Pot,
Stéphanie Guyomard-Rabenirina,
David Couvin,
Célia Ducat,
Vincent Enouf,
Séverine Ferdinand,
Gaëlle Gruel,
Edith Malpote,
Antoine Talarmin,
Sébastien Breurec,
Yann Reynaud
Publication year - 2021
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.02146-20
Subject(s) - enterobacter cloacae , plasmid , west indies , lineage (genetic) , enterobacteriaceae infections , enterobacteriaceae , caribbean region , biology , microbiology and biotechnology , genetics , escherichia coli , history , gene , ethnology , linguistics , philosophy , latin americans
Wastewater treatment plants are considered hot spots for antibiotic resistance. Most studies have addressed the impact on the aquatic environment, as water is an important source of anthropogenic pollutants. Few investigations have been conducted on terrestrial animals living near treatment ponds. We isolated extended-spectrum-β-lactamase Enterobacter cloacae complex-producing strains from 35 clinical isolates, 29 samples of wastewater, 19 wild animals, and 10 domestic animals living in the hospital sewers and at or near a wastewater treatment plant to study the dissemination of clinically relevant resistance through hospital and urban effluents. After comparison of the antibiotic-resistant profiles of E. cloacae complex strains, a more detailed analysis of 41 whole-genome-sequenced strains demonstrated that the most common sequence type, ST114 ( n = 20), was present in human ( n = 9) and nonhuman ( n = 11) samples, with a close genetic relatedness. Whole-genome sequencing confirmed local circulation of this pathogenic lineage in diverse animal species. In addition, nanopore sequencing and specific synteny of an IncHI2/ST1/ bla CTX-M-15 plasmid recovered on the majority of these ST114 clones ( n = 18) indicated successful worldwide diffusion of this mobile genetic element.