Open AccessCharacterization of a Plasmid-Encoded Resistance-Nodulation-Division Efflux Pump in Klebsiella pneumoniae and Klebsiella quasipneumoniae from Patients in China
Author(s) -
Ruowen He,
Yongfu Yang,
Yichao Wu,
LanLan Zhong,
Yanxian Yang,
Guanping Chen,
Mingli Qin,
Xingxing Liang,
Mohamed Abd El-Gawad El-Sayed Ahmed,
Minmin Lin,
Bin Yan,
Yong Xia,
Min Dai,
Hongtao Chen,
GuoBao Tian
Publication year - 2021
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.02075-20
Subject(s) - klebsiella pneumoniae , tigecycline , microbiology and biotechnology , efflux , klebsiella , multiple drug resistance , klebsiella infections , biology , escherichia coli , plasmid , enterobacteriaceae , drug resistance , antibiotics , virology , gene , genetics
Two multidrug-resistant (MDR) mcr-1 -harboring Klebsiella pneumoniae isolates from patients with urinary tract infections and one MDR Klebsiella quasipneumoniae isolate from a patient with bloodstream infection were identified to carry tmexCD1-toprJ1 The addition of the efflux pump inhibitor reduced the tigecycline MIC against all three isolates by 8- to 16-fold. pKQBSI104-1 was transferred from K. quasipneumoniae to Escherichia coli J53 via conjugation. The tmexCD1-toprJ1 -carrying plasmids pKP15ZE495-1 (102,569 bp) and pKQBSI104-1 (121,996 bp) were completely sequenced and analyzed.