Open AccessIdentification and Characterization of Two Novel IS CR1 -Associated Genes, dfrA42 and dfrA43 , Encoding Trimethoprim-Resistant Dihydrofolate Reductases
Author(s) -
Ling Li,
Mengge Zhang,
Wenjia Wang,
Ruirui Xia,
Yong Ma,
Wei Xin,
Xinhua Wang,
Xiaomin Sun,
Xiulan Xie,
Shiling Xie,
Mingyu Wang,
Hai Xu
Publication year - 2021
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.02010-20
Subject(s) - dihydrofolate reductase , gene , trimethoprim , genetics , identification (biology) , biology , folic acid antagonists , computational biology , antibiotics , immunology , methotrexate , botany
Two novel IS CR1 -associated dfr genes, dfrA42 and dfrA43 , were identified from trimethoprim (TMP)-resistant Proteus strains and were shown to confer high level TMP resistance (MIC ≥ 1024 mg/L) when cloned into Escherichia coli These genes were hosted by complex class 1 integrons suggesting their potentials for dissemination. Analysis of enzymatic parameters and TMP affinity were performed, suggesting that the mechanism of TMP resistance for these novel DHFRs is the reduction of binding with TMP.