Open AccessIn Vitro Activity of Eravacycline against Gram-Positive Bacteria Isolated in Clinical Laboratories Worldwide from 2013 to 2017
Author(s) -
Ian Morrissey,
Stephen Hawser,
Sibylle Lob,
James A. Karlowsky,
Matteo Bassetti,
G. Ralph Corey,
Melanie Olesky,
Joseph Newman,
Corey Fyfe
Publication year - 2020
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01715-19
Subject(s) - broth microdilution , microbiology and biotechnology , enterococcus , antimicrobial , biology , gram positive bacteria , antibiotics , gram , bacteria , minimum inhibitory concentration , genetics
Eravacycline is a novel, fully synthetic fluorocycline antibiotic being developed for the treatment of serious infections, including those caused by resistant Gram-positive pathogens. Here, we evaluated the in vitro activities of eravacycline and comparator antimicrobial agents against a recent global collection of frequently encountered clinical isolates of Gram-positive bacteria. The CLSI broth microdilution method was used to determine in vitro MIC data for isolates of Enterococcus spp. ( n = 2,807), Staphylococcus spp. ( n = 4,331), and Streptococcus spp. ( n = 3,373) isolated primarily from respiratory, intra-abdominal, urinary, and skin specimens by clinical laboratories in 37 countries on three continents from 2013 to 2017. Susceptibilities were interpreted using both CLSI and EUCAST breakpoints. There were no substantive differences (a >1-doubling-dilution increase or decrease) in eravacycline MIC 90 values for different species/organism groups over time or by region. Eravacycline showed MIC 50 and MIC 90 results of 0.06 and 0.12 μg/ml, respectively, when tested against Staphylococcus aureus , regardless of methicillin susceptibility. The MIC 90 values of eravacycline for Staphylococcus epidermidis and Staphylococcus haemolyticus were equal (0.5 μg/ml). The eravacycline MIC 90 s for Enterococcus faecalis and Enterococcus faecium were 0.06 μg/ml and were within 1 doubling dilution regardless of the vancomycin susceptibility profile. Eravacycline exhibited MIC 90 results of ≤0.06 μg/ml when tested against Streptococcus pneumoniae and beta-hemolytic and viridans group streptococcal isolates. In this surveillance study, eravacycline demonstrated potent in vitro activity against frequently isolated clinical isolates of Gram-positive bacteria ( Enterococcus , Staphylococcus , and Streptococcus spp.), including isolates collected over a 5-year period (2013 to 2017), underscoring its potential benefit in the treatment of infections caused by common Gram-positive pathogens.