Open AccessfosM , a New Family of Fosfomycin Resistance Genes Identified in Bacterial Species Isolated from Human Microbiota
Author(s) -
Sami Khabthani,
Mouna Hamel,
Sophie Alexandra Baron,
Seydina M. Diène,
JeanMarc Rolain,
Vicky Merhej
Publication year - 2021
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01712-20
Subject(s) - fosfomycin , microbiology and biotechnology , in silico , bacteria , biology , antibiotics , antibiotic resistance , in vitro , gene , genetics
Fosfomycin is a decades-old antibiotic, currently reused because of its activity against multidrug-resistant bacteria. Here, we used a combined in vitro/in silico approach to search for fosfomycin resistance determinants in 25 new bacterial species isolated from the human microbiota. Putative resistance genes were cloned into a susceptible Escherichia coli strain. MIC values increased from 1 μg/ml to 1,024 μg/ml. Here, we report a new family of potential chromosomal fosfomycin resistance genes, named fosM .