Open AccessThe Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial Genus Shinella
Author(s) -
Nicolas Kieffer,
Stefan Ebmeyer,
D. G. Joakim Larsson
Publication year - 2020
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01263-20
Subject(s) - biology , gene , homology (biology) , escherichia coli , gene duplication , genetics , homologous chromosome , enterobacteriaceae , microbiology and biotechnology , comparative genomics , genome , genomics
Comparative genomics identified the environmental bacterial genus Shinella as the most likely origin of the class A carbapenemases BKC-1 and GPC-1. Available sequences and PCR analyses of additional Shinella species revealed homologous β-lactamases showing up to 85.4% and 93.3% amino acid identity to both enzymes, respectively. The genes conferred resistance to β-lactams once expressed in Escherichia coli bla BKC-1 likely evolved from a putative ancestral Shinella gene with higher homology through duplication of a gene fragment.