Open AccessComparative Analysis of Plasmodium falciparum Genotyping via SNP Detection, Microsatellite Profiling, and Whole-Genome Sequencing
Author(s) -
Mariko Kanai,
Tomas Yeo,
Victor Asua,
Philip J. Rosenthal,
David A. Fidock,
Sachel Mok
Publication year - 2022
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01163-21
Subject(s) - genotyping , biology , plasmodium falciparum , microsatellite , genome , genetics , typing , snp genotyping , computational biology , single nucleotide polymorphism , molecular inversion probe , snp , snp array , whole genome sequencing , genotype , malaria , gene , allele , immunology
Research efforts to combat antimalarial drug resistance rely on quick, robust, and sensitive methods to genetically characterizePlasmodium falciparum parasites. We developed a single-nucleotide polymorphism (SNP)-based genotyping method that can assess 33 drug resistance-conferring SNPs indhfr, dhps, pfmdr1, pfcrt, andk13 in nine PCRs, performed directly fromP. falciparum cultures or infected blood.