Open AccessIdentification and Characterization of a Novel FosA7 Member from Fosfomycin-Resistant Escherichia coli Clinical Isolates from Canadian Hospitals
Author(s) -
Kieran A. Milner,
Denice C. Bay,
David C. Alexander,
Andrew Walkty,
James A. Karlowsky,
Michael R. Mulvey,
Meenu K. Sharma,
George G. Zhanel
Publication year - 2020
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00865-20
Subject(s) - fosfomycin , escherichia coli , microbiology and biotechnology , biology , gene , antibiotic resistance , identification (biology) , antimicrobial , genetics , computational biology , antibiotics , botany
Here, we characterize the fosA genes from three Escherichia coli clinical isolates recovered from Canadian patients. Each fosA sequence was individually overexpressed in E. coli BW25113, and antimicrobial susceptibility testing was performed to assess their role in fosfomycin resistance. The findings from this study identify and functionally characterize FosA3, FosA8, and novel FosA7 members and highlight the importance of phenotypic characterization of fosA genes.