Open AccessIS Kpn40 -Mediated Mobilization of the Colistin Resistance Gene mcr-3.11 in Escherichia coli
Author(s) -
Yuzhang He,
Tengfei Long,
Caiping Chen,
Bing He,
Xingping Li,
Jessica A. Schuyler,
Liang Chen,
XiaoPing Liao,
Yahong Liu,
Jian Sun
Publication year - 2020
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00851-20
Subject(s) - colistin , escherichia coli , mcr 1 , transposable element , transposition (logic) , gene , biology , microbiology and biotechnology , mobile genetic elements , genetics , plasmid , enterobacteriaceae , antibiotics , mutant , linguistics , philosophy
The mobile colistin resistance gene mcr-3 has globally disseminated since it was first reported in 2017 in Escherichia coli In vitro mobilization assays in this study demonstrate the functionality of the composite transposon structure IS Kpn40-mcr-3.11-dgkA -IS Kpn40 in wild-type and recA - E. coli strains. These transpositions generated 4-bp duplications at the target sites. This is the first report demonstrating the mobility of the mcr-3.11 gene by transposition.