Open AccessCharacterization of Large Deletion Mutants of Mycobacterium tuberculosis Selected for Isoniazid Resistance
Author(s) -
Catherine Vilchèze,
Rajagopalan Saranathan,
Brian Weinrick,
William R. Jacobs
Publication year - 2020
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00792-20
Subject(s) - isoniazid , mycobacterium tuberculosis , tuberculosis , mutant , intracellular , oxidative stress , microbiology and biotechnology , biology , gene , in vitro , phenotype , genetics , biochemistry , medicine , pathology
Large genomic deletions (LGDs) (6 to 63 kbp) were observed in isoniazid-resistant Mycobacterium tuberculosis mutants derived from four M. tuberculosis strains. These LGDs had no growth defect in vitro but could be defective in intracellular growth and showed various sensitivities toward oxidative stress despite lacking katG The LGD regions comprise 74 genes, mostly of unknown function, that may be important for M. tuberculosis intracellular growth and protection against oxidative stress.