Open AccessRibosome-Mediated Attenuation of vga (A) Expression Is Shaped by the Antibiotic Resistance Specificity of Vga(A) Protein Variants
Author(s) -
Vladimir Vimberg,
Jorunn Pauline Cavanagh,
Michaela Novotná,
Jakub Lenart,
Bich Nguyen Thi Ngoc,
Jana Veselá,
Maria Pain,
Markéta Koběrská,
Gabriela Balíková Novotná
Publication year - 2020
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00666-20
Subject(s) - lincosamides , video graphics array , biology , ribosome , translation (biology) , genetics , rna , antibiotics , computational biology , antibiotic resistance , gene , messenger rna , chip , electrical engineering , engineering
Vga(A) protein variants confer different levels of resistance to lincosamides, streptogramin A, and pleuromutilins (LS A P) by displacing antibiotics from the ribosome. Here, we show that expression of vga (A) variants from Staphylococcus haemolyticus is regulated by cis -regulatory RNA in response to the LS A P antibiotics by the mechanism of ribosome-mediated attenuation. The specificity of induction depends on Vga(A)-mediated resistance rather than on the sequence of the riboregulator. Fine tuning between Vga(A) activity and its expression in response to the antibiotics may contribute to the selection of more potent Vga(A) variants because newly acquired mutation can be immediately phenotypically manifested.