Open AccessCandida glabrata Transcription Factor Rpn4 Mediates Fluconazole Resistance through Regulation of Ergosterol Biosynthesis and Plasma Membrane Permeability
Author(s) -
Pedro Pais,
Raquel Califórnia,
Mónica Galocha,
Romeu Viana,
Mihaela Ola,
Mafalda Cavalheiro,
Azusa TakahashiNakaguchi,
Hiroji Chibana,
Géraldine Butler,
Miguel C. Teixeira
Publication year - 2020
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00554-20
Subject(s) - ergosterol , candida glabrata , fluconazole , azole , biology , transcription factor , microbiology and biotechnology , gene , biochemistry , antifungal
The ability to acquire azole resistance is an emblematic trait of the fungal pathogen Candida glabrata . Understanding the molecular basis of azole resistance in this pathogen is crucial for designing more suitable therapeutic strategies. This study shows that the C. glabrata transcription factor (TF) CgRpn4 is a determinant of azole drug resistance. RNA sequencing during fluconazole exposure revealed that CgRpn4 regulates the expression of 212 genes, activating 80 genes and repressing, likely in an indirect fashion, 132 genes.