Open AccessPathogenicity Genomic Island-Associated CrpP-Like Fluoroquinolone-Modifying Enzymes among Pseudomonas aeruginosa Clinical Isolates in Europe
Author(s) -
José Manuel Ortiz de la Rosa,
Patrice Nordmann,
Laurent Poirel
Publication year - 2020
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00489-20
Subject(s) - pseudomonas aeruginosa , ciprofloxacin , microbiology and biotechnology , biology , plasmid , bacteria , gene , antibiotics , genetics
Many transferable quinolone resistance mechanisms have been identified in Gram-negative bacteria. The plasmid-encoded 65-amino-acid-long ciprofloxacin-modifying enzyme CrpP was recently identified in Pseudomonas aeruginosa isolates. We analyzed a collection of 100 clonally unrelated and multidrug-resistant P. aeruginosa clinical isolates, among which 46 were positive for crpP -like genes, encoding five CrpP variants conferring variable levels of reduced susceptibility to fluoroquinolones. These crpP -like genes were chromosomally located as part of pathogenicity genomic islands.