Open AccessIn VitroProfiling of Antitubercular Compounds by Rapid, Efficient, and Nondestructive Assays Using Autoluminescent Mycobacterium tuberculosis
Author(s) -
Gauri Shetye,
Kyung Bae Choi,
Chang-Yub Kim,
Scott G. Franzblau,
Sang Hyun Cho
Publication year - 2021
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00282-21
Subject(s) - mycobacterium tuberculosis , in vitro , tuberculosis , microbiology and biotechnology , minimum bactericidal concentration , minimum inhibitory concentration , chemistry , biology , antimicrobial , medicine , biochemistry , pathology
Anti-infective drug discovery is greatly facilitated by the availability ofin vitro assays that are more proficient at predicting the preclinical success of screening hits. Tuberculosis (TB) drug discovery is hindered by the relatively slow growth rate ofMycobacterium and the use of whole-cell-basedin vitro assays that are inherently time-consuming, and for these reasons, rapid, noninvasive bioluminescence-based assays have been widely used in anti-TB drug discovery and development. In this study,in vitro assays that employ autoluminescentMycobacterium tuberculosis .