Open AccessEvidence for Polyadenylated mRNA in Pseudomonas aeruginosa
Author(s) -
Senthil S. Saravanamuthu,
Franz von Götz,
Prabhakar Salunkhe,
Chozhavendan Rathinam,
Robert Geffers,
Jan Buer,
Burkhard Tümmler,
Ivo Steinmetz
Publication year - 2004
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.186.20.7015-7018.2004
Subject(s) - biology , pseudomonas aeruginosa , complementary dna , oligonucleotide , dna microarray , polyadenylation , microbiology and biotechnology , gene , dna , messenger rna , nucleic acid thermodynamics , gene expression , genetics , rna , bacteria
In this paper, we report the synthesis of Pseudomonas aeruginosa cDNA in the presence of oligo(dT) primers. Hybridization of oligonucleotide DNA microarrays indicates that under the experimental conditions used, at least 43.7% of the expressed genes from P. aeruginosa PAO1, representing many different functional classes, can be detected by using oligo(dT)-primed cDNAs.