Open AccessDetection of N -(3-Oxohexanoyl)- l -Homoserine Lactone in Mice Infected with Yersinia enterocolitica Serotype O8
Author(s) -
Christoph A. Jacobi,
A. Bach,
Leo Eberl,
Anette Steidle,
Jürgen Heesemann
Publication year - 2003
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.71.11.6624-6626.2003
Subject(s) - yersinia enterocolitica , biology , microbiology and biotechnology , serotype , escherichia coli , virulence , homoserine , enterobacteriaceae , yersinia , lactone , yersinia infections , bacteria , quorum sensing , gene , biochemistry , genetics
Yersinia enterocolitica synthesizes N-acyl-L-homoserine lactone (AHL) signal molecules via the LuxR-LuxI homologues YenR-YenI. In this study we checked two prototypes of mouse-virulent Y. enterocolitica serotype O8 strains WA-314 and 8081 for AHL production in vitro and in vivo (mouse infection model). We used thin-layer chromatography in combination with the Escherichia coli AHL biosensor to identify the AHL species produced. We detected only OHHL [N-(3-oxohexanoyl)-L-homoserine lactone] and not HHL (N-hexanoyl-L-homoserine lactone) produced by Y. enterocolitica O8 in culture supernatant or infected mouse tissue. This is the first report demonstrating AHL production by yersiniae during infection.