Direct observation of motor protein stepping in living cells using MINFLUX | Zendy

Takahiro Deguchi | Zendy; Malina K. Iwanski | Zendy; Eva-Maria Schentarra | Zendy; Christopher Heidebrecht | Zendy; L.D. Schmidt | Zendy; Jennifer Heck | Zendy; Tobias Weihs | Zendy; Sebastian Schnorrenberg | Zendy; Philipp Hoess | Zendy; Sheng Liu | Zendy; Veronika Chevyreva | Zendy; KyungMin Noh | Zendy; Lukas C. Kapitein | Zendy; Jonas Ries | Zendy

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Direct observation of motor protein stepping in living cells using MINFLUX

Author(s) -

Takahiro Deguchi,

Malina K. Iwanski,

Eva-Maria Schentarra,

Christopher Heidebrecht,

L.D. Schmidt,

Jennifer Heck,

Tobias Weihs,

Sebastian Schnorrenberg,

Philipp Hoess,

Sheng Liu,

Veronika Chevyreva,

KyungMin Noh,

Lukas C. Kapitein,

Jonas Ries

Publication year - 2023

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.ade2676

Subject(s) - millisecond , microtubule , motor protein , kinesin , molecular motor , tracking (education) , resolution (logic) , temporal resolution , biological system , cytoskeleton , nanotechnology , nanoscopic scale , computer science , biophysics , physics , biology , materials science , microbiology and biotechnology , cell , artificial intelligence , optics , psychology , pedagogy , genetics , astronomy

Dynamic measurements of molecular machines can provide invaluable insights into their mechanism, but these measurements have been challenging in living cells. Here, we developed live-cell tracking of single fluorophores with nanometer spatial and millisecond temporal resolution in two and three dimensions using the recently introduced super-resolution technique MINFLUX. Using this approach, we resolved the precise stepping motion of the motor protein kinesin-1 as it walked on microtubules in living cells. Nanoscopic tracking of motors walking on the microtubules of fixed cells also enabled us to resolve the architecture of the microtubule cytoskeleton with protofilament resolution.

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