Open AccessMechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation
Author(s) -
Qian Wang,
Yan Xue,
Laixing Zhang,
Zhenhui Zhong,
Suhua Feng,
Changshi Wang,
Lifan Xiao,
Zhenlin Yang,
C. Jake Harris,
Zhe Wu,
Jixian Zhai,
Maojun Yang,
Sisi Li,
Steven E. Jacobsen,
Jiamu Du
Publication year - 2021
Publication title -
science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 12.556
H-Index - 1186
eISSN - 1095-9203
pISSN - 0036-8075
DOI - 10.1126/science.abl4546
Subject(s) - dicer , mechanism (biology) , wafer dicing , microbiology and biotechnology , rna , small interfering rna , rna interference , chemistry , rna silencing , biology , genetics , gene , physics , organic chemistry , layer (electronics) , quantum mechanics
Ready to dice In the biogenesis of small RNAs, the Dicer family endonucleases act as a molecular ruler to cut the substrate RNA into defined lengths. Wanget al . report a structure of the dicing-competent state of plant DICER LIKE PROTEIN 3 (DCL3) in complex with a pre–small interfering RNA (pre-siRNA). Toward one end of the pre-siRNA, DCL3 uses a positively charged pocket and an aromatic cap to specifically recognize the 5′-phosphorylated adenosine of the guide strand and the 3′ overhang of the complementary strand, respectively. On the other end, the paired ribonuclease III domains of DCL3 cut both strands of the RNA, determining the precise length of the product small RNA. —DJ
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