Open AccessMIC-Drop: A platform for large-scale in vivo CRISPR screens
Author(s) -
Saba Parvez,
Chelsea Herdman,
Manu Beerens,
Korak Chakraborti,
Zachary P. Harmer,
Jing-Ruey Joanna Yeh,
Calum A. MacRae,
H. Joseph Yost,
Randall T. Peterson
Publication year - 2021
Publication title -
science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 12.556
H-Index - 1186
eISSN - 1095-9203
pISSN - 0036-8075
DOI - 10.1126/science.abi8870
Subject(s) - crispr , biology , genetic screen , computational biology , gene , drop (telecommunication) , genome editing , cas9 , ribonucleoprotein , microfluidics , genome , genetics , phenotype , rna , computer science , nanotechnology , telecommunications , materials science
Screen time for CRISPR CRISPR-Cas9 has been used to edit the genomes of organisms ranging from fruit flies to primates, but it has not been used in large-scale genetic screens in animals because generating, validating, and keeping track of large numbers of mutant animals is prohibitively laborious. Parvezet al . have developed Multiplexed Intermixed CRISPR Droplets, or MIC-Drop, a platform combining droplet microfluidics, en masse CRISPR injections, and barcoding, to enable large-scale genetic screens. In pilot phenotypic screens in zebrafish, MIC-Drop enabled rapid identification of the target of a small molecule and discovery of several new genes governing cardiovascular development. MIC-Drop is potentially scalable to thousands of targets and adaptable to diverse organisms and experiments. —DJ
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