Open AccessStructure of the human Mediator-bound transcription preinitiation complex
Author(s) -
R. Abdella,
Anna Talyzina,
Siyu Chen,
Carla Inouye,
Robert Tjian,
Yuan He
Publication year - 2021
Publication title -
science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 12.556
H-Index - 1186
eISSN - 1095-9203
pISSN - 0036-8075
DOI - 10.1126/science.abg3074
Subject(s) - transcription preinitiation complex , mediator , transcription factor ii f , transcription factor ii e , rna polymerase ii , rna polymerase ii holoenzyme , eukaryotic transcription , transcription factor ii d , microbiology and biotechnology , general transcription factor , transcription (linguistics) , transcription factor ii a , biology , gene , computational biology , genetics , rna , promoter , gene expression , rna polymerase , linguistics , philosophy
Mediating transcription The Mediator complex is recruited by transcription factors to all protein-coding genes in eukaryotes and helps to assemble the machinery necessary to transcribe the gene. Abdellaet al. present the cryo–electron microscopy structure of the human Mediator-bound preinitiation complex (Med-PIC). The structure shows how Mediator positions the long, flexible C-terminal domain of RNA polymerase II to be phosphorylated by the kinase CDK7, a crucial step for further processing of the RNA into a mature RNA. Most sites where transcription factors bind to Mediator are flexibly tethered to the complex, allowing the large Med-PIC to assemble at any gene.Science , this issue p.52
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