In vivo Perturb-Seq reveals neuronal and glial abnormalities associated with autism risk genes | Zendy

Xin Jin | Zendy; Sean Simmons | Zendy; Amy X. Guo | Zendy; Ashwin S. Shetty | Zendy; Michelle Ko | Zendy; Lan Nguyễn | Zendy; Vahbiz Jokhi | Zendy; Elise Robinson | Zendy; Paul Oyler | Zendy; Nathan Curry | Zendy; Giulio Deangeli | Zendy; Simona Lodato | Zendy; Joshua Z. Levin | Zendy; Aviv Regev | Zendy; Feng Zhang | Zendy; Paola Arlotta | Zendy

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In vivo Perturb-Seq reveals neuronal and glial abnormalities associated with autism risk genes

Author(s) -

Xin Jin,

Sean Simmons,

Amy X. Guo,

Ashwin S. Shetty,

Michelle Ko,

Lan Nguyễn,

Vahbiz Jokhi,

Elise Robinson,

Paul Oyler,

Nathan Curry,

Giulio Deangeli,

Simona Lodato,

Joshua Z. Levin,

Aviv Regev,

Feng Zhang,

Paola Arlotta

Publication year - 2020

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.aaz6063

Subject(s) - biology , gene , frameshift mutation , genetics , crispr , cell type , autism , phenotype , model organism , cell , computational biology , medicine , psychiatry

An in vivo analysis of autism risk genes CRISPR targeting in vivo, especially in mammals, can be difficult and time consuming when attempting to determine the effects of a single gene. However, such studies may be required to identify pathological gene variants with effects in specific cells along a developmental trajectory. To study the function of genes implicated in autism spectrum disorders (ASDs), Jinet al. applied a gene-editing and single-cell–sequencing system, Perturb-Seq, to knock out 35 ASD candidate genes in multiple mice embryos (see the Perspective by Treutlein and Camp). This method identified networks of gene expression in neuronal and glial cells that suggest new functions in ASD-related genes.Science , this issue p.eaaz6063 ; see also p.1038

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