RNA polymerase II–associated factor 1 regulates the release and phosphorylation of paused RNA polymerase II | Zendy

Ming Yu | Zendy; Wenjing Yang | Zendy; Ting Ni | Zendy; Zhanyun Tang | Zendy; Tomoyoshi Nakadai | Zendy; Jun Zhu | Zendy; Robert G. Roeder | Zendy

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RNA polymerase II–associated factor 1 regulates the release and phosphorylation of paused RNA polymerase II

Author(s) -

Ming Yu,

Wenjing Yang,

Ting Ni,

Zhanyun Tang,

Tomoyoshi Nakadai,

Jun Zhu,

Robert G. Roeder

Publication year - 2015

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.aad2338

Subject(s) - rna polymerase ii , transcription factor ii f , transcription factor ii e , transcription factor ii d , rna polymerase iii , transcription (linguistics) , polymerase , rna polymerase ii holoenzyme , transcription factor ii b , rna polymerase i , microbiology and biotechnology , promoter , chemistry , biology , gene , rna dependent rna polymerase , genetics , gene expression , linguistics , philosophy

Release of promoter-proximal paused RNA polymerase II (Pol II) during early elongation is a critical step in transcriptional regulation in metazoan cells. Paused Pol II release is thought to require the kinase activity of cyclin-dependent kinase 9 (CDK9) for the phosphorylation of DRB sensitivity-inducing factor, negative elongation factor, and C-terminal domain (CTD) serine-2 of Pol II. We found that Pol II-associated factor 1 (PAF1) is a critical regulator of paused Pol II release, that positive transcription elongation factor b (P-TEFb) directly regulates the initial recruitment of PAF1 complex (PAF1C) to genes, and that the subsequent recruitment of CDK12 is dependent on PAF1C. These findings reveal cooperativity among P-TEFb, PAF1C, and CDK12 in pausing release and Pol II CTD phosphorylation.

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