Open AccessAcetylation of Metabolic Enzymes Coordinates Carbon Source Utilization and Metabolic Flux
Author(s) -
Qijun Wang,
Yakun Zhang,
Chen Yang,
Huiming Xiong,
Yan Lin,
Jun Yao,
Hong Li,
Lu Xie,
Wei Zhao,
Yufeng Yao,
Zhi Bin Ning,
Rong Zeng,
Yue Xiong,
Kun Liang Guan,
Shimin Zhao,
Guo Ping Zhao
Publication year - 2010
Publication title -
science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 12.556
H-Index - 1186
eISSN - 1095-9203
pISSN - 0036-8075
DOI - 10.1126/science.1179687
Subject(s) - acetylation , metabolic pathway , enzyme , biochemistry , lysine , flux (metallurgy) , metabolism , metabolic control analysis , biology , function (biology) , chemistry , gene , microbiology and biotechnology , amino acid , organic chemistry , insulin , endocrinology
Lysine acetylation regulates many eukaryotic cellular processes, but its function in prokaryotes is largely unknown. We demonstrated that central metabolism enzymes in Salmonella were acetylated extensively and differentially in response to different carbon sources, concomitantly with changes in cell growth and metabolic flux. The relative activities of key enzymes controlling the direction of glycolysis versus gluconeogenesis and the branching between citrate cycle and glyoxylate bypass were all regulated by acetylation. This modulation is mainly controlled by a pair of lysine acetyltransferase and deacetylase, whose expressions are coordinated with growth status. Reversible acetylation of metabolic enzymes ensure that cells respond environmental changes via promptly sensing cellular energy status and flexibly altering reaction rates or directions. It represents a metabolic regulatory mechanism conserved from bacteria to mammals.