Open AccessTb 3+ -doped fluorescent glass for biology
Author(s) -
Kazuki Okamoto,
Teppei Ebina,
Naoki Fujii,
Kuniaki Konishi,
Yu Sato,
Tetsuhiko Kashima,
Risako Nakano,
Hiroyuki Hioki,
Haruki Takeuchi,
Junji Yumoto,
Masunori Matsuzaki,
Yuji Ikegaya
Publication year - 2021
Publication title -
science advances
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.928
H-Index - 146
ISSN - 2375-2548
DOI - 10.1126/sciadv.abd2529
Subject(s) - fluorescence , borosilicate glass , green fluorescent protein , electroporation , materials science , terbium , doping , pipette , photon upconversion , biophysics , nanotechnology , optoelectronics , luminescence , chemistry , optics , biology , biochemistry , physics , composite material , gene
Optical investigation and manipulation constitute the core of biological experiments. Here, we introduce a new borosilicate glass material that contains the rare-earth ion terbium(III) (Tb 3+ ), which emits green fluorescence upon blue light excitation, similar to green fluorescent protein (GFP), and thus is widely compatible with conventional biological research environments. Micropipettes made of Tb 3+ -doped glass allowed us to target GFP-labeled cells for single-cell electroporation, single-cell transcriptome analysis (Patch-seq), and patch-clamp recording under real-time fluorescence microscopic control. The glass also exhibited potent third harmonic generation upon infrared laser excitation and was usable for online optical targeting of fluorescently labeled neurons in the in vivo neocortex. Thus, Tb 3+ -doped glass simplifies many procedures in biological experiments.