Premium
Optical simulations of a noninvasive technique for the diagnosis of diseased salivary glands in situ
Author(s) -
Gannot I.,
Bonner R. F.,
Gannot G.,
Fox P. C.,
Smith P. D.,
Gandjbakhche A. H.
Publication year - 1998
Publication title -
medical physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.473
H-Index - 180
eISSN - 2473-4209
pISSN - 0094-2405
DOI - 10.1118/1.598305
Subject(s) - fluorophore , scattering , fluorescence , imaging phantom , salivary gland , optics , absorption (acoustics) , materials science , light scattering , biomedical engineering , chemistry , biophysics , physics , biology , medicine , biochemistry
A simulation experiment for three‐dimensional (3D) imaging of exogenous fluorescinated antibodies that specifically bind to infiltrating lymphocytes in minor salivary glands was carried out. Small ( ∼ 1 mm 3volume) rhodamine targets, which mimic diseased minor salivary glands labeled with fluorescent antibodies to infiltrating lymphocytes in Sjo/gren's syndrome, were embedded in a highly scattering tissue phantom consisting of a thick Delrin™ disk covered by index matched Delrin™ slabs of various thickness. In this way the variation of fluorescence profiles on the surface of tissue could be examined corresponding to the range of depths of the salivary glands in vivo . Surface images were obtained for different target depths and radial distances from laser excitation to target fluorophore. These images were analyzed and compared to calculations based on random walk theory in turbid media, using previously determined scattering and absorption coefficients of the Delrin™. Excellent agreement between the surface profiles experimentally measured and those predicted by our random walk theory was obtained. Derivation of these theoretical expressions is a necessary step toward devising an inverse algorithm which may have the potential expressions to perform 3D reconstruction of the concentration distribution of fluorescent labels within tissue.