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In vivo 31‐P NMR of photoactivated hematoporphyrin derivative in cat brain
Author(s) -
Chopp Michael,
Helpern J. A.,
Frinak Stanley,
Hetzel Fred W.,
Ewing James R.,
Welch K. M. A.
Publication year - 1985
Publication title -
medical physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.473
H-Index - 180
eISSN - 2473-4209
pISSN - 0094-2405
DOI - 10.1118/1.595765
Subject(s) - phosphocreatine , hematoporphyrin , in vivo , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , chemistry , nucleoside triphosphate , adenosine triphosphate , biophysics , biochemistry , energy metabolism , biology , medicine , stereochemistry , nucleotide , photodynamic therapy , physics , microbiology and biotechnology , organic chemistry , gene
In vivo 31‐P nuclear magnetic resonance (NMR) spectroscopy was performed on cat brains injected with hematoporphyrin derivative (HpD). A 2‐cm‐diam region of the right parietal lobe was photoactivated with red light. The 31‐P NMR spectra of the photoactivated hemisphere revealed increased inorganic phosphate and decreased phosphocreatine and adenosine triphosphate (ATP) levels, compared to spectra obtained from the control hemisphere. In the absence of drug, no difference in spectra was observed between the photoradiated and control lobes. Our studies suggest that in vivo 31‐P NMR spectroscopy may be used to monitor the effects of phototherapy on tissue high‐energy phosphate metabolism.