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Whole body hyperthermia (41–42 °C): A simple technique for unanesthetized mice
Author(s) -
Robins H. Ian,
Steeves Richard A.,
Shecterle Linda M.,
Martin Patricia A.,
Miller K. A.,
Paliwal Bhudatt,
Neville Alan J.,
Dennis Warren H.
Publication year - 1984
Publication title -
medical physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.473
H-Index - 180
eISSN - 2473-4209
pISSN - 0094-2405
DOI - 10.1118/1.595571
Subject(s) - hyperthermia , radiant heat , nuclear medicine , rectal temperature , core temperature , chemistry , zoology , thermoregulation , biomedical engineering , materials science , medicine , anesthesia , biology , composite material
A technique for accomplishing 41–42 °C whole body hyperthermia (WBH) in unrestrained, unanesthetized mice using a simple apparatus is reported. This method combines a radiant heat technology with monitoring of individual rectal temperatures at 10‐min intervals. In 66 heating sessions, involving 116 AKR mice and a total of 619 WBH treatments, the mortality rate was less than 1%. Treatment at 41–42 °C for periods of up to 120 min were accomplished. Relationships of several variables of mouse temperature–time profiles were studied including (1) initial core temperature, (2) rate of heating, (3) whole body irradiation, and (4) the presence of tumor (transplanted AKR leukemia). Sham treatments produce a consistent temperature–time profile showing about a 1 °C rise. Between‐mouse variability, as well as between‐treatment variability in individual animals was estimated. Thermal mapping of the device demonstrates a range from 32–38 °C of the air temperature in the zone with the mice to a high of 47 °C near to the radiant heating surface at the top of the apparatus.