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Sci‐Thur PM – Colourful Interactions: Highlights 03: Radiation induced glycogen accumulation in non‐small cell lung cancer xenografts detected using Raman spectroscopy
Author(s) -
Harder Samantha J.,
Isabelle Martin,
DeVorkin Lindsay,
Smazynski Julian,
Beckham Wayne,
Brolo Alexandre,
Lum Julian,
Jirasek Andrew
Publication year - 2016
Publication title -
medical physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.473
H-Index - 180
eISSN - 2473-4209
pISSN - 0094-2405
DOI - 10.1118/1.4961763
Subject(s) - glycogen , immunohistochemistry , in vivo , pathology , raman spectroscopy , lung cancer , radiation therapy , irradiation , staining , biology , cancer research , nuclear medicine , medicine , biochemistry , optics , physics , microbiology and biotechnology , nuclear physics
Purpose: This study presents the novel application of Raman spectroscopy (RS) to identify biochemical signatures of radiation response in human non‐small cell lung cancer (NSCLC) xenografts, irradiated in vivo . Methods: Human NSCLC cells (H460) were subcutaneously injected into the flanks of 12 mice. Tumours were treated with single fraction radiation doses (0, 5 or 15 Gy) and harvested at 3 days post irradiation. A Renishaw inVia Raman microscope coupled to a 785 nm laser was used to collect Raman spectral maps for each tumour. Immunohistochemistry (IHC) staining for CAIX was used to visualize hypoxia, and co‐registration between IHC fluorescence and Raman images was carried out. Results: Principal component analysis revealed radiation induced spectral signatures linked to changes in protein, nucleic acid, lipid and carbohydrates. In particular, a marked increase in glycogen for irradiated tumours was observed. Spatial mapping revealed intra‐tumoural heterogeneity in the distribution of glycogen within the tumour, suggesting tumour response to radiation is not globally uniform. Furthermore, co‐registration of Raman glycogen maps with CAIX IHC staining showed a correlation between glycogen rich and hypoxic regions of the tissue. Conclusions: We identify glycogen as a unique radiation induced response in NSCLC tumour xenografts, which may reflect inherent metabolic changes associated with radiation response in tissue. This study provides unique insight into the biochemical response of tumours, irradiated in vivo , and demonstrates the potential of RS for detecting radiobiological responses in tumours.