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SU‐F‐J‐215: Non‐Thermal Pulsed High Intensity Focused Ultrasound Therapy Combined with 5‐Aminolevulinic Acid: An in Vivo Pilot Study
Author(s) -
Wang B,
He W,
Cvetkovic D,
Chen L,
Ma C
Publication year - 2016
Publication title -
medical physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.473
H-Index - 180
eISSN - 2473-4209
pISSN - 0094-2405
DOI - 10.1118/1.4956123
Subject(s) - sonodynamic therapy , medicine , nuclear medicine , ultrasound , in vivo , cancer , radiology , biology , microbiology and biotechnology
Purpose: It has recently been shown that non‐thermal pulsed high intensity focused ultrasound (pHIFU) has a cell‐killing effect. The purpose of the study is to investigate the sonosensitizing effect of 5‐Aminolevulinic Acid (5‐ALA) in non‐thermal pHIFU cancer therapy. Methods: FaDu human head and neck squamous cell carcinoma cells were injected subcutaneously in the flanks of nude mice. After one to two weeks, the tumors reached the volume of 112 ± 8 mm3 and were assigned randomly into a non‐thermal pHIFU group (n=9) and a non‐thermal sonodynamic therapy (pHIFU after 5‐ALA administration) group (n=7). The pHIFU treatments (parameters: 1 MHz frequency; 25 W acoustic power; 0.1 duty cycle; 60 seconds duration) were delivered using an InSightec ExAblate 2000 system with a GE Signa 1.5T MR scanner. The mice in the non‐thermal sonodynamic group received 5‐ALA tail‐vein injection 4 hours prior to the pHIFU treatment. The tumor growth was monitored using the CT scanner on a Sofie‐Biosciences G8 PET/CT system. Results: The tumors in this study grew very aggressively and about 60% of the tumors in this study developed ulcerations at various stages. Tumor growth delay after treatments was observed by comparing the treated (n=9 in pHIFU group; n=7 in sonodynamic group) and untreated tumors (n=17). However, no statistically significant differences were found between the non‐thermal pHIFU and non‐thermal sonodynamic group. The mean normalized tumor volume of the untreated tumors on Day 7 after their first CT scans was 7.05 ± 0.54, while the normalized volume of the treated tumors on Day 7 after treatment was 5.89 ± 0.79 and 6.27 ± 0.47 for the sonodynamic group and pHIFU group, respectively. Conclusion: In this study, no significant sonosensitizing effects of 5‐ALA were obtained on aggressive FaDu tumors despite apparent tumor growth delay in some mice treated with non‐thermal sonodynamic therapy.