Open AccessA method for validating depth-resolved biodistributions in topically-stained specimen with multi-channel fluorescence cryo-imaging
Author(s) -
Brook K. Byrd,
Rendall S. Strawbridge,
Wendy A. Wells,
Connor W. Barth,
Summer L. Gibbs,
Scott C. Davis
Publication year - 2021
Publication title -
pubmed central
Language(s) - English
Resource type - Conference proceedings
SCImago Journal Rank - 0.192
H-Index - 176
pISSN - 0277-786X
DOI - 10.1117/12.2582542
Subject(s) - staining , biomedical engineering , biomarker , fluorescence lifetime imaging microscopy , fluorescence , pathology , materials science , medicine , chemistry , optics , biochemistry , physics
Fluorescent contrast agents targeted to cancer biomarkers are increasingly being explored for cancer detection, surgical guidance, and response monitoring. Efforts have been underway to topically apply such biomarker-targeted agents to freshly excised specimen for detecting cancer cell receptors on the surface as a method for intraoperative surgical margin assessment, including dual-probe staining methods introduce a second 'non-specific' optical agent as a control to help compensate for heterogeneous uptake and normalize the imaging field. Still, such specimen staining protocols introduce multifaceted complexity with unknown variables, such as tissue-specific diffusion, cell-specific binding and disassociation rates, and other factors, affecting the interpreted cancer-biomarker distribution across the specimen surface. The ability to recover three-dimensional dual-probe biodistributions throughout whole-specimens could offer a ground-truth validation method for examining topical staining uptake behaviors. Herein, we report on a novel method for characterizing dual-probe accumulation with 3D depth-profiles observed from a dual-probe fresh-specimen staining experiment.