Open AccessDeveloping a novel hyperspectral imaging cryomacrotome for whole body fluorescence imaging
Author(s) -
Boyu Meng,
Brook K. Byrd,
Dennis J. Wirth,
Rendall R. Strawbridge,
Scott C. Davis
Publication year - 2020
Publication title -
pubmed central
Language(s) - English
Resource type - Conference proceedings
SCImago Journal Rank - 0.192
H-Index - 176
pISSN - 0277-786X
DOI - 10.1117/12.2544497
Subject(s) - hyperspectral imaging , autofluorescence , fluorescence , fluorescence lifetime imaging microscopy , voxel , whole body imaging , computer science , nuclear magnetic resonance , biomedical engineering , artificial intelligence , optics , nuclear medicine , physics , positron emission tomography , medicine
The ability to directly measure whole-body fluorescence can enable tracking of labeled cells, metastatic spread, and drug bio-distribution. We describe the development of a new hyperspectral imaging whole body cryo-macrotome designed to acquire 3-D fluorescence volumes in large specimens (whole animals) at high resolution. The use of hyperspectral acquisition provides full spectra at every voxel, enabling spectral decoupling of multiple fluorohpores and autofluorescence. We present examples of tissue spectra and spectral fitting in a rodent glioma xenograft.