Open AccessFabrication of nanoscale “curtain rods” for DNA curtains using nanoimprint lithography
Author(s) -
Teresa Fazio,
Mari-Liis Visnapuu,
Eric C. Greene,
Shalom J. Wind
Publication year - 2009
Publication title -
journal of vacuum science and technology. b, microelectronics and nanometer structures
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 112
eISSN - 1520-8567
pISSN - 1071-1023
DOI - 10.1116/1.3259951
Subject(s) - nanoimprint lithography , nanotechnology , materials science , nanoscopic scale , bilayer , lithography , dna origami , microfluidics , dna , fabrication , nanostructure , membrane , chemistry , optoelectronics , medicine , biochemistry , alternative medicine , pathology
We have developed a new lithographically-based patterning process which significantly increases the throughput of experiments which probe how repair proteins scan DNA molecules for errors. In this process, nanoscale barriers are formed to interrupt the flow of a lipid bilayer in which DNA is tethered to proteins in the bilayer. The barriers trap the DNA, which is then stretched out by hydrodynamic flow, resulting in the formation of "DNA curtains." Nanoimprint lithography is used to facilitate massively parallel data collection for protein diffusion experiments on DNA.