Open AccessAnálisis del gen dhaT en cepas Colombianas de Clostridium sp. (Clostridia) productoras de 1,3-propanodiol.
Author(s) -
Diana Milena Quilaguy-Ayure,
José David Montoya-Solano,
Zulma Rocío Suárez-Moreno,
José Mauricio Bernal-Morales,
Dolly Montoya-Castaño
Publication year - 2010
Publication title -
universitas scientiarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.207
H-Index - 10
eISSN - 2027-1352
pISSN - 0122-7483
DOI - 10.11144/javeriana.sc15-1.atdg
Subject(s) - operon , clostridia , clostridium butyricum , gene , clostridium , strain (injury) , biology , chemistry , genetics , escherichia coli , bacteria , anatomy
Objective: to analyze the dhaT gene, one of the genes responsible for the 1,3-propanediol (1,3-PD) production, in two native Clostridium strains. Materials and methods: The dhaT gene was amplified by Polimerase Chain Reaction with specific primers designed from Clostridium butyricum VPI1718 operon. Bioinformatics tools like BLASTN, ORF finder, BLASTP and ClustalW were used to determine the identity of the sequence and to assign a function. Results: DNA amplification products were obtained from Colombian Clostridium sp. native strains (IBUN 13A and IBUN 158B) and the Clostridium butyricum DSM 2478 strain, which were sequenced. According to the bioinformatics analysis of the above sequences, a high degree of similarity was found with the dhaT gene of different bacterial species. The highest percentage of identity was obtained with the Clostridium butyricum VPI 1718 strain. Conclusion: knowledge of the physical structure of the 1,3-PD operon in native strains opens the way for developing genetic and metabolic engineering strategies for improving processes productivity.Key words: 1,3-propanediol, 1,3-propanediol dehydrogenase, dhaT gene, 1,3-propanediol operon.