Open AccessEFFECTS OF LIGHT-EMITTING DIODE (LED 640NM) ON HUMAN GINGIVAL FIBROBLASTS: A COMPARATIVE IN VITRO STUDY
Author(s) -
P M Mandrillo,
G Fischetto,
P Odorisio,
Francesca Maria Curà,
Anna Avantaggiato,
Francesco Carinci
Publication year - 2017
Publication title -
oral and implantology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.298
H-Index - 17
eISSN - 2035-2468
pISSN - 1974-5648
DOI - 10.11138/orl/2017.10.2.151
Subject(s) - elastin , fibroblast , trypsinization , microbiology and biotechnology , wound healing , chemistry , in vitro , biology , immunology , pathology , medicine , trypsin , biochemistry , enzyme
The light-emitting diodes (LEDs) have been applied in oral surgery for tissue stimulation and wound healing. Several Authors have highlighted that fibroblasts subjected to phototherapy have an increased viability, proliferation, biomodulation of inflammatory cytokines and genes expression. It remains to be determined which are the best irradiation parameters (energy, wavelength, power) for each type of cell in order to obtain the best bio-stimulation. The aim of this study was to investigate the effects of LED irradiation on primary human gingival fibroblast cells (HGF) on DSP, ELN, HAS1, ELANE, HYAL1, RPL13 genes activation using Real Time PCR. These genes activation is directly connected with elastin protein production and HGF functionality.