Open AccessOPTIMIZATION OF PROCESS PARAMETERS FOR CULTIVATION OF BACTERIOPHAGES PROMISING FOR BIOLOGICAL CONTROL OF PHYTOPATHOGENIC BACTERIA OF GENUS PSEUDOMONAS
Author(s) -
Т. А. Пилипчук,
А. Д. Герасимович,
I. N. Ananyeva,
Э. И. Коломиец
Publication year - 2021
Publication title -
eurasian journal of applied biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2617-1147
pISSN - 2617-1139
DOI - 10.11134/btp.3.2021.3
Subject(s) - lytic cycle , pseudomonas , bacteria , strain (injury) , aeration , microbiology and biotechnology , pseudomonas syringae , lysis , hydrolysate , food science , biology , chemistry , virus , biochemistry , virology , ecology , hydrolysis , genetics , anatomy
Cultural parameters of bacteriophages screened for lytic activity toward host bacterial strain P. helmanticensis BIM В-582 D were investigated. The following conditions were shown to be optimal for cultivation of indicator culture and it’s lysis by Pseudomonas phages BIM ВV-45 D, BIM ВV-46 D, BIM ВV-47 D, BIM ВV-50 D, BIM ВV-53 D, BIM ВV-61 D in laboratory fermenters ANKUM-3M: nutrient medium – fish meal hydrolysate broth, temperature – 28°C, stirrer agitation rate – 160 rpm, aeration rate – 1 l air/ l medium per min. Biopreparation produced under optimized conditions and composed of six afore-mentioned phages display high lytic activity both in regard to the host strain and to phytopathogenic bacteria Pseudomonas syringae.