Open AccessBuccal Dna Collection Using Treated Paper
Author(s) -
S. Atavliyeva,
P. Tarlykov
Publication year - 2020
Publication title -
eurasian journal of applied biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2617-1147
pISSN - 2617-1139
DOI - 10.11134/btp.1.2020.9
Subject(s) - buccal swab , buccal administration , genotyping , dna , nucleic acid , hypervariable region , multiplex , microsatellite , microbiology and biotechnology , biology , lysis , dna extraction , polymerase chain reaction , genetics , genotype , gene , bioinformatics , allele
This study presents a domestic kit suitable for the collection of human buccal cells, and long-term storage of nucleic acids on a piece of paper. A filter paper with a density of 70 g/m2 was selected and treated with chemicals that make up the lysing solution, which contributes to the destruction of cells and prevents bacterial growth. A total of 25 buccal cell samples were collected on the treated paper cards. The samples had a median DNA concentration of 20 ng/μl (range 0.5–45 ng/μl). The collected DNA samples were tested using a real-time PCR, sequencing of the first hypervariable segment of the human mtDNA control region, and multiplex genotyping of 15 short tandem repeat loci. We conclude that the domestic pretreated cards could be used for collecting and isolating DNA from buccal epithelium for genetic and other applications.