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Atom‐by‐atom engineering of voltage‐gated ion channels: Magnified insights into function and pharmacology
Author(s) -
Pless Stephan A.,
Kim Robin Y.,
Ahern Christopher A.,
Kurata Harley T.
Publication year - 2015
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2014.287714
Subject(s) - amino acid , ion channel , function (biology) , mutagenesis , chemistry , ion , atom (system on chip) , voltage gated ion channel , resolution (logic) , biophysics , computational biology , biochemistry , biology , computer science , genetics , mutation , gene , receptor , organic chemistry , artificial intelligence , embedded system
Unnatural amino acid incorporation into ion channels has proven to be a valuable approach to interrogate detailed hypotheses arising from atomic resolution structures. In this short review, we provide a brief overview of some of the basic principles and methods for incorporation of unnatural amino acids into proteins. We also review insights into the function and pharmacology of voltage‐gated ion channels that have emerged from unnatural amino acid mutagenesis approaches.