Signalling to contractile proteins by muscarinic and purinergic pathways in neurally stimulated bladder smooth muscle

Key points•  Parasympathetic nerves release the neurotransmitters ATP and acetylcholine that activate purinergic and muscarinic receptors, respectively, to initiate contraction of urinary bladder smooth muscle. •  Although both receptors mediate Ca 2+ influx for myosin regulatory light chain (RLC) phosphorylation necessary for contraction, the muscarinic receptor may also recruit cellular mechanisms affecting the Ca 2+ sensitivity of RLC phosphorylation. •  Using transgenic mice expressing Ca 2+ /calmodulin sensor myosin light chain kinase (MLCK) in smooth muscles, the effects of selective purinergic or muscarinic receptor inhibition were examined on neurally stimulated tissues in relation to signalling pathways converging on RLC phosphorylation. •  Purinergic‐mediated Ca 2+ signals provide the initial Ca 2+ /calmodulin activation of MLCK with muscarinic receptors supporting sustained responses. •  Activation of muscarinic receptors leads phosphorylation of myosin light chain phosphatase inhibitor CPR‐17 to enhance Ca 2+ sensitivity while also initiating phosphorylation‐dependent Ca 2+ desensitization of MLCK. The interplay between Ca 2+ sensitization and desensitization mechanisms fine tunes the contractile signalling module for force development.Abstract  Urinary bladder smooth muscle contraction is triggered by parasympathetic nerves, which release ATP and acetylcholine (ACh) that bind to purinergic and muscarinic receptors, respectively. Neuronal signalling may thus elicit myosin regulatory light chain (RLC) phosphorylation and contraction through the combined, but distinct contributions of these receptors. Both receptors mediate Ca 2+ influx whereas muscarinic receptors may also recruit Ca 2+ ‐sensitization mechanisms. Using transgenic mice expressing calmodulin sensor myosin light chain kinase (MLCK) in smooth muscles, the effects of suramin/α,β‐methyleneATP (α,β‐meATP) (purinergic inhibition) or atropine (muscarinic inhibition) on neurally stimulated elevation of [Ca 2+ ] i , MLCK activation, force and phosphorylation of RLC, myosin light chain phosphatase (MLCP) targeting subunit MYPT1 and MLCP inhibitor protein CPI‐17 were examined. Electric field stimulation (EFS) increased [Ca 2+ ] i , MLCK activation and concomitant force in a frequency‐dependent manner. The dependence of force on [Ca 2+ ] i and MLCK activation decreased with time suggesting increased Ca 2+ sensitization in the late contractile phase. RLC and CPI‐17 phosphorylation increased upon stimulation with maximal responses at 20 Hz; both responses were attenuated by atropine, but only RLC phosphorylation was inhibited by suramin/α,β‐meATP. Antagonism of purinergic receptors suppressed maximal MLCK activation to a greater extent in the early contractile phase than in the late contractile phase; atropine had the opposite effect. A frequency‐ and time‐dependent increase in MLCK phosphorylation explained the desensitization of MLCK to Ca 2+ , since MLCK activation declined more rapidly than [Ca 2+ ] i . EFS elicited little or no effect on MYPT1 Thr696 or 850 phosphorylation. Thus, purinergic Ca 2+ signals provide the initial activation of MLCK with muscarinic receptors supporting sustained responses. Activation of muscarinic receptors recruits CPI‐17, but not MYPT1‐mediated Ca 2+ sensitization. Furthermore, nerve‐released ACh also initiates signalling cascades leading to phosphorylation‐dependent desensitization of MLCK.