Lack of an endogenous GABA A receptor‐mediated tonic current in hypoglossal motoneurons

Key points•  Tonic GABA A receptor‐mediated currents have profound effects on neuronal excitability, yet it is not known whether this current is present in mammalian motoneurons. •  This study shows that tonic GABA A receptor‐mediated current can be observed in hypoglossal motoneurons in vitro under certain experimental conditions. •  The tonic current was only observed when exogenous GABA was applied and GABA transporters were blocked suggesting that GABA transporters highly regulate extracellular GABA concentration. •  Furthermore, we demonstrate that the current probably arises from activation of extrasynaptic GABA A receptors containing a δ subunit. •  This tonic current may function to reduce the excitability of hypoglossal motoneurons; these motoneurons are important in many functions including chewing, swallowing, suckling, vocalization and upper airway patency.Abstract  Tonic GABA A receptor‐mediated current is an important modulator of neuronal excitability, but it is not known if it is present in mammalian motoneurons. To address this question studies were performed using whole‐cell patch‐clamp recordings from mouse hypoglossal motoneurons (HMs) in an in vitro slice preparation. In the presence of blockers of glutamatergic and glycinergic receptor‐mediated transmission application of SR‐95531 or bicuculline, while abolishing GABA A receptor‐mediated phasic synaptic currents, did not reveal a tonic GABA A receptor‐mediated current. Additionally, blockade of both GAT‐1 and GAT‐3 GABA transporters did not unmask this tonic current. In contrast, application of exogenous GABA (1 to 15 μ m ) resulted in a tonic GABAergic current that was observed when both GAT‐1 and GAT‐3 transporters were simultaneously blocked, and this current was greater than the sum of the current observed when each transporter was blocked individually. We also investigated which GABA A receptor subunits may be responsible for the current. Application of the δ subunit GABA A receptor agonist THIP resulted in a tonic GABA A receptor current. Application of the δ subunit modulator THDOC resulted in an enhanced tonic current. Application of the α 5 subunit GABA A receptor inverse agonist L‐655,708 did not modulate the current. In conclusion, these data show that HMs have tonic GABA A receptor‐mediated current. The level of GABA in the vicinity of GABA A receptors responsible for this current is regulated by GABA transporters. In HMs a tonic current in response to exogenous GABA probably arises from activation of GABA A receptors containing δ subunits.