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Distinct contributions by ionotropic purinoceptor subtypes to ATP‐evoked calcium signals in mouse parotid acinar cells
Author(s) -
Bhattacharya Sumit,
Verrill Douglas S.,
Carbone Kristopher M.,
Brown Stefanie,
Yule David I.,
Giovannucci David R.
Publication year - 2012
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2012.228148
Subject(s) - ionotropic effect , purinergic receptor , ion channel , exocytosis , microbiology and biotechnology , transient receptor potential channel , neuroscience , chemistry , biology , receptor , endocrinology , biochemistry , extracellular , glutamate receptor , secretion
Key points• There are two major ionotropic purinoceptor subtypes (ATP‐gated, non‐selective, Ca 2+ ‐conducting ion channels) in the salivary gland. • Relatively little is known about the physiological roles of these purinoceptors regarding compartmentalization and selective activation, their contributions to the spatiotemporal properties of intracellular Ca 2+ signals and their roles in regulating protein exocytosis and ion channel activity. • In this study, we investigated the subtype‐specific sub‐cellular distribution and functional characterization of purinoceptors in mouse parotid acinar cells. • Selective activation of ionotropic purinergic receptor subtypes was shown to evoke spatially distinct cytosolic Ca 2+ signals as well as protein exocytosis. • This study identifies a subtype of ionotropic purinergic receptors as a potential therapeutic target for treatment of salivary gland hypofunction.