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Differential expression and function of nicotinic acetylcholine receptors in the urinary bladder epithelium of the rat
Author(s) -
Beckel Jonathan M.,
Birder Lori A.
Publication year - 2012
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2011.226860
Subject(s) - urothelium , purinergic receptor , acetylcholine , ppads , stimulation , receptor , nicotinic agonist , urinary bladder , endocrinology , chemistry , medicine , p2 receptor , urothelial cell , biology
Key points • It has been previously shown that stimulation of urothelial nicotinic acetylcholine receptors (nAChRs) can alter reflex bladder activity in the rat; the current study examines this further. • Stimulation of rat urothelial cells with an α7 nAChR agonist increases intracellular calcium through internal stores and decreases basal ATP release. • Stimulation with an α3* nAChR agonist increases intracellular calcium through extracellular influx and increases basal ATP release. • Infusion of an α3* agonist into the bladder lumen of the rat increases reflex bladder activity, which is blocked by intra‐arterial administration of a purinergic antagonist. • The cellular effects of α3* stimulation are blocked when the cells are pretreated with an α7 agonist, suggesting cross‐talk between the receptors: this cross‐talk may be mediated through protein kinase A or protein kinase C.