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A method for detecting molecular transport within the cerebral ventricles of live zebrafish ( Danio rerio ) larvae
Author(s) -
Turner Maxwell H.,
Ullmann Jeremy F.P.,
Kay Alan R.
Publication year - 2012
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2011.225896
Subject(s) - danio , zebrafish , anatomy , biology , cerebral ventricle , lateral ventricles , left ventricles , microbiology and biotechnology , medicine , biochemistry , cardiology , gene , ventricle
Key points • The cerebral ventricles of the vertebrate brain form a series of interconnected chambers through which cerebrospinal fluid flows serving an important role in brain homeostasis and development. • We introduce the larval zebrafish brain as a model for studying the physiology of the cerebral ventricles. • The three dimensional form of the zebrafish ventricles was characterized by in vivo confocal microscopy and found to be similar to that of mammals. • To follow the movement of molecules within ventricles we have a developed a technique using the uncaging of a fluorescent molecule. • Zebrafish larvae provide a tractable model for studying ventricles and the movement of chemicals across the blood–brain barrier.