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Purinergic neuromuscular transmission is absent in the colon of P2Y 1 knocked out mice
Author(s) -
Gallego Diana,
Gil Víctor,
MartínezCutillas Míriam,
Mañé Noemí,
Martín Maria Teresa,
Jiménez Marcel
Publication year - 2012
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2011.224345
Subject(s) - purinergic receptor , neurotransmission , neuromuscular transmission , hyperpolarization (physics) , p2y receptor , depolarization , medicine , endocrinology , inhibitory postsynaptic potential , ppads , nad+ kinase , chemistry , stimulation , receptor , biology , biochemistry , organic chemistry , nuclear magnetic resonance spectroscopy , enzyme
Key points • Neural‐mediated relaxation occurs in the gastrointestinal tract. To accomplish this function, two neurotransmitters, ATP or a related purine and nitric oxide, are released by inhibitory motorneurons. • The type of purinergic receptor is still under debate but previous data using a classical pharmacological approach (receptor agonists and antagonists) suggested that P2Y 1 receptors are responsible for purinergic neurotransmission in the gastrointestinal tract. • In the present study we used a genetically modified mouse in which P2Y 1 receptors had been knocked out. • P2Y 1 ‐deficient mice had functional nitrergic neurotransmission but purinergic neurotransmission was absent. • The present work confirms the hypothesis demonstrating that P2Y 1 receptors mediate the purinergic component of the smooth muscle relaxation in the gastrointestinal tract.