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Advanced in vitro approach to study neurovascular coupling mechanisms in the brain microcirculation
Author(s) -
Kim Ki Jung,
Filosa Jessica A.
Publication year - 2012
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2011.222778
Subject(s) - neurovascular bundle , microcirculation , neuroscience , in vitro , coupling (piping) , medicine , chemistry , psychology , anatomy , engineering , mechanical engineering , biochemistry
Key points • We show that parenchymal arterioles of less than 30 μm in diameter can be successfully cannulated and perfused in cortical and hypothalamic brain slices. • Increased flow/pressure induced parenchymal arteriolar constriction. • Alternatively to video microscopy, diameter measurements of cannulated arterioles could be obtained from perfusion velocity values obtained using fluorescence recovery after photobleaching. • Perfused and pressurized parenchymal arterioles responded with dilatation to two well‐established signals in neurovascular coupling, K + (10 mM) and activation of metabotropic glutamate receptors (mGluRs).