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Non‐invasive long‐term and real‐time analysis of endocrine cells on micro‐electrode arrays
Author(s) -
Raoux Matthieu,
Bornat Yannick,
Quotb Adam,
Catargi Bogdan,
Renaud Sylvie,
Lang Jochen
Publication year - 2012
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2011.220038
Subject(s) - enteroendocrine cell , glucose homeostasis , hormone , endocrine system , homeostasis , islet , neuroscience , function (biology) , electrophysiology , biology , diabetes mellitus , microbiology and biotechnology , endocrinology , medicine , insulin resistance
Key points • Pancreatic islet cells are required for glucose homeostasis and their dysfunction leads to diabetes mellitus. • The electrical activity of these cells is regulated by nutrients as well as hormones and this provides the first integrative read‐out reflecting their function. • Non‐invasive recording of their electrical activity and its analysis in real time would provide the possibility of long‐term and repetitive investigations or testing of multiple drugs. • Combining electrophysiology and microelectronics, we now demonstrate such an approach with multiple (up to 60) electrodes and including on‐line signal analysis on the two major cell types present in islets, α‐ and β‐cells. • This method should be applicable to other endocrine cells and may serve in the long‐run as the basis for a novel biosensor.