Premium
Chronic imaging of cortical sensory map dynamics using a genetically encoded calcium indicator
Author(s) -
Minderer Matthias,
Liu Wenrui,
Sumanovski Lazar T.,
Kügler Sebastian,
Helmchen Fritjof,
Margolis David J.
Publication year - 2012
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2011.219014
Subject(s) - sensory system , calcium imaging , neuroscience , cerebral cortex , calcium , cortical neurons , cortex (anatomy) , biology , medicine
Non‐technical summary Optical imaging is widely used to map functional areas of the cerebral cortex. We present a method for fast fluorescence imaging of map‐level cortical activity using a calcium indicator protein. Sensory‐evoked neuronal activity can be imaged repeatedly in the same mouse over weeks, enabling new opportunities for the longitudinal study of cortical function and dysfunction. We hope this method will be flexibly applied across different cortical areas and to a variety of newly developed genetically encoded calcium and voltage sensors.