Premium
Kinetic changes in tetanic Ca 2+ transients in enzymatically dissociated muscle fibres under repetitive stimulation
Author(s) -
Calderón Juan C.,
Bolaños Pura,
Caputo Carlo
Publication year - 2011
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2011.213314
Subject(s) - chemistry , stimulation , kinetics , biophysics , muscle fibre , time constant , anatomy , skeletal muscle , endocrinology , biology , physics , engineering , quantum mechanics , electrical engineering
Non‐Technical Summary The transient and progressive decrease in skeletal muscle performance during contraction is known as fatigue. One of the phenomena associated with fatigue is an alteration in the excitation–contraction coupling mechanism. Using isolated muscle fibres loaded with the fast Ca 2+ dye Magfluo‐4, we have found that after a protocol of repetitive stimulation there are alterations in the ability of the fibres to release and reuptake Ca 2+ , which are more evident and rapidly established in fast fibres compared to slow ones. All alterations were reversed after several minutes of rest and were not related to a phenomenon of inactivation of Ca 2+ release. These data increase our knowledge of the events present during muscle fatigue and will help understand the mechanisms responsible for them.